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High-throughput nucleotide sequence analysis of diverse bacterial communities in leachates of decomposing pig carcasses Genet. Mol. Biol.
Yang,Seung Hak; Lim,Joung Soo; Khan,Modabber Ahmed; Kim,Bong Soo; Choi,Dong Yoon; Lee,Eun Young; Ahn,Hee Kwon.
The leachate generated by the decomposition of animal carcass has been implicated as an environmental contaminant surrounding the burial site. High-throughput nucleotide sequencing was conducted to investigate the bacterial communities in leachates from the decomposition of pig carcasses. We acquired 51,230 reads from six different samples (1, 2, 3, 4, 6 and 14 week-old carcasses) and found that sequences representing the phylum Firmicutes predominated. The diversity of bacterial 16S rRNA gene sequences in the leachate was the highest at 6 weeks, in contrast to those at 2 and 14 weeks. The relative abundance of Firmicutes was reduced, while the proportion of Bacteroidetes and Proteobacteria increased from 3–6 weeks. The representation of phyla was restored...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pig; Decomposition; Leachate; Bacterial community; Pyrosequencing.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300373
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